Abstract
Phytoliths are important plant microfossils, but also relevant components of the clastic fraction of the soils. The phytolith pool present in soils not only depends on plant phytolith production but also on taphonomical soil processes. In the southeastern Pampean Plains, Argentina, where typical Argiudolls dominate, intense agricultural and livestock activities are carried on. These managements affect both plant diversity and soil properties, which in turn, may influence on the soil phytolith assemblages. The aim of this study was to describe and compare the phytolith assemblages of soils under different land uses and to compare them with the current phytolith production of plants. Three plots with different land uses were selected (pasture, cultivated field, and nature reserve). Phytoliths were extracted from plants and dung by calcination. Soil phytoliths were extracted using a centrifugation technique and the total soil phytolith content was determined. Phytolith production was higher in crops than in non-crop species. Differences in the distribution and content of phytoliths were found in soils under different land uses and no close relationship was observed between current vegetation and soil phytoliths. Soils in the nature reserve, where living plants are trace phytolith producers, had the highest phytolith content values. Cropland soils had the lowest phytolith content and the highest degradation state. The results showed that land use leads to differences in phytolith content in soils. Agricultural management modifies the typical pattern of phytolith distribution along profiles and reduces the total content of phytoliths, whereas nature plots with plant invasion modify the accumulation of organic debris in soils and promote a higher preservation of phytoliths. Finally, dung introduces both new and degraded phytoliths into soils.
References
Allan, E., Manning, P., Alt, F., Binkenstein, J., Blaser, S., Blüthgen, N., Fischer, M. et al. (2015). Land use intensification alters ecosystem multifunctionality via loss of biodiversity and changes to functional composition. Ecology Letters, 18(8), 834 – 843. https://doi.org/10.1111/ele.12469
Álvarez, F., Osterrieth, M., Bernava Laborde, V., & Montti, L. (2008). Evaluación de propiedades de agregados en Argiudoles típicos del sudeste bonaerense sometidos a distintos usos del suelo. Revista Argentina de la Ciencia del suelo, 26(2), 115–129.
Alvarez, M. F., Osterrieth, M., & del Río, J. L. (2012). Changes on aggregates morphology and roughness of induced by different uses of typical Argiudolls, Buenos Aires province, Argentina. Soil & Tillage Research, 119, 38–49. https://doi.org/10.1016/j.still.2011.12.003
Alvarez, M. F., Osterrieth, M., & Cooper, M. (2018). Changes in the porosity induced by tillage in typical Argiudolls of southeastern Buenos Aires Province, Argentina, and its relationship with the living space of the mesofauna: a preliminary study. Environmental Earth Sciences, 77(4), 134. https://doi.org/10.1007/s12665-018-7313-x
Bardgett, R. D., Bullock, J. M., Lavorel, S., Manning, P., Schaffner, U., Ostle, N., Chomel, M., Durigan, G., Fry, E. L., Johnson, D., Lavallee, J. M., Le Provost, G., Luo, S., Png, K., Sankaran, M., Hou, X., Zhou, H., Ma, L., Ren, W., Li, X., Ding, Y., Li, Y. & Shi, H., et al. (2021). Combatting global grassland degradation. Nature Reviews Earth & Environment, 2(10), 720–735. https://doi.org/10.1038/s43017-021-00207-2
Bellora, G., Lozano, L. A., Soracco, C. G., Guilino, F., Polich, N., Salazar, M. P., Villarreal, R., & Palancar, T. (2023). Tránsito repetido sobre la misma huella: efecto en las propiedades fisicas de un argiudol típico. Ciencia del Suelo, 41(1).
Benvenuto, M. L. (2017). Silicofitolitos en especies dominantes de pastizales, cultivos y suelos asociados en el sudeste bonaerense [Tesis doctoral]. Facultad de Ciencias Exactas y Naturales. Universidad Nacional de Mar del Plata.
Benvenuto, M. L., De Rito, M., Osterrieth, M. L., & Fernández Honaine, M. (2025). Analysis of phytolith inputs from natural plant communities and crops and soil silicon availability (Southeastern Pampean region, Argentina). Flora, 322, 152640. https://doi.org/10.1016/j.flora.2024.152640
Bertoldi de Pomar, H. (1975). Synopsis of the knowledge of silicophytoliths [in plants]. Darwiniana.
Blinnikov, M. S. (2005). Phytoliths in plants and soils of the interior Pacific Northwest, USA. Review Palaeobotany and Palynology, 135. 71– 98.
Borrelli, N., Osterrieth, M., & Marcovecchio, J. (2008). Interrelations of vegetal cover, silicophytolith content and pedogenesis of Typical Argiudolls of the Pampean Plain, Argentina. Catena, 146–153. https://doi.org/10.1016/j.catena.2008.05.001
Borrelli, N., Álvarez, M. F., Osterrieth, M., & Marcovecchio, J. E. (2010). Silica content in soil solution and its relation with phytolith weathering and silica biogeochemical cycle in Typical Argiudolls of the Pampean Plain, Argentina: a Preliminary Study. Journal of Soils and Sediments, 983–994. https://doi.org/10.1007/s11368-010-0205-7
Borrelli, N., Fernández Honaine, M., Altamirano, S., & Osterrieth, M. (2011). Calcium and silica biomineralizations in species associated to aquatic environments of the Pampean Plain, Argentina. Aquatic Botany, 94(1), 29–36. https://doi.org/10.1016/j.aquabot.2010.10.003
Burgos, J. J., & Vidal, A. (1951). Los climas de la República Argentina según la nueva clasificación de Thornthwaite. Meteoros, 1(1), 3–32.
Cabanes, D., Weiner, S., & Shahack-Gross, R. (2011). Stability of phytoliths in the archaeological record: a dissolution study of modern and fossil phytoliths. Journal of Archaeological Science, 38(9), 2480–2490. https://doi.org/10.1016/j.jas.2011.05.020
Carey, J. C., & Fulweiler, R. W. (2012). Human activities directly alter watershed dissolved silica fluxes. Biogeochemistry, 111, 125–138. https://doi.org/10.1007/s10533-011-9671-2
De Rito, M. (2015). Relación entre las asociaciones fitolíticas de especies vegetales y suelos asociados a talares en el sudeste de la provincia de Buenos Aires [Tesis de grado]. Universidad Nacional de Mar del Plata.
De Rito, M., Fernandez Honaine, M., & Osterrieth, M. (2018). Silicophytoliths from a Pampean native tree community (Celtis ehrenbergiana community) and their representation in the soil assemblage. Review Palaeobotany and Palynology, 257, 19–34. https://doi.org/10.1016/j.revpalbo.2018.06.002
Desplanques, V., Cary, L., Mouret, J. C., Trolard, F., Bourrie, G., Grauby, O., & Meunier, J. D. (2006). Silicon transfers in a rice field in Camargue (France). Journal of Geochemical Exploration, 88, 190–193. https://doi.org/10.1016/j.gexplo.2005.08.036
Duval, M. E., Galantini, J. A., Martínez, J. M., & Limbozzi, F. (2018). Labile soil organic carbon for assessing soil quality: influence of management practices and edaphic conditions. Catena, 171, 316–326. https://doi.org/10.1016/j.catena.2018.07.023
Fahmy, A. G. (2008). Diversity of lobate phytoliths in grass leaves from the Sahel region, West Tropical Africa: Tribe Paniceae. Plant Systematics and Evolution, 270, 1–23. https://doi.org/10.1007/s00606-007-0597-z
Fernández Honaine, M. (2001). Estudio de la relación entre geomorfología, suelo y vegetación de la Reserva Integral Laguna de Los Padres Buenos Aires: Un instrumento para la gestión de manejo [Tesis de grado]. Universidad Nacional de Mar del Plata.
Fernández Honaine, M., Zucol, A. F., & Osterrieth, M. (2006). Phytolith Assemblages and Systematic Associations in Grassland Species of the South-Eastern Pampean Plains, Argentina. Annals of Botany, 98, 1155–1165. https://doi.org/10.1093/aob/mcl207
Fernández Honaine, M. (2007). Análisis fitolítico del pastizal de Paspalum quadrifarium y su relación con la evolución pedológica en el sudeste de la provincia de Buenos Aires [Ph. D. tesis]. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Argentina.
Fernández Honaine, M., Bernava Laborde, V., & Zucol, A. (2008). Contenido de sílice en gramíneas del pastizal nativo del sudeste bonaerense. In A. Korstanje, & P. Babot, (Ed.), Matices interdisciplinarios en estudios fitolíticos y de otros microfósiles (pp. 57–63). BAR International Series S1870, Oxford, Inglaterra.
Fernández Honaine, M., Osterrieth, M., & Zucol, A. (2009). Plant communities and soil phytolith assemblages relationship in native grasslands from southeastern Buenos Aires Province, Argentina. Catena, 76, 89–96. https://doi.org/10.1016/j.catena.2008.09.011
Fraysse, F., Pokrovsky, O. S., Schott, J., & Meunier, J. D. (2009). Surface chemistry and reactivity of plant phytoliths in aqueous solutions. Chemical Geology, 258(3-4), 197–206. https://doi.org/10.1016/j.chemgeo.2008.10.003
Fredlund, G. G., & Tieszen, L. T. (1994). Modern phytolith assemblages from the North American Great Plains. Journal of Biogeography, 21, 312–335. https://doi.org/10.2307/2845533
Galehouse, J. S. (1971). Sedimentation Analysis. In Carver (Ed.), Procedures in Sedimentary Petrology (69–94). Wisconsin, USA, Wiley Interscience.
Gallego, L., & Distel, R. A. (2004). Phytolith assemblages in grasses native to Central Argentina. Annals of Botany, 94, 865–874. https://doi.org/10.1093/aob/mch214
Hart, D. M., & Humphreys, G. S. (2003). Phytolith depth functions in surface regolith materials. Advances in Regolith, CRC LEME, 159–163.
Haynes, R. J. (2017). Significance and role of Si in crop production. Advances Agronomy, 146, 83–166. https://doi.org/10.1016/bs.agron.2017.06.001
Hodson, M. J., White, P. J., Mead, A., Broadley, M. R. (2005). Phylogenetic variation in the silicon composition of plants. Annals of Botany, 96(6), 1027–1046. https://doi.org/10.1093/aob/mci255
International Committee for Phytolith Taxonomy (K. Neumann, C. A. E. Strömberg,T. Ball, R. M. Albert, L. Vrydaghs & L. Scott Cummings), (2019). International Code for Phytolith Nomenclature (ICPN) 2.0. Annals of Botany, 124(2), 189–199. https://doi.org/10.1093/aob/mcz064
Ingram, R.L. (1971). Sieve Analysis. In R. E., Carver (Ed.) Procedures in Sedimentary Petrology (pp. 41–68). Wisconsin, USA, Wiley Interscience.
INTA (1989). Mapa de suelos de la provincia de Buenos Aires, Esc. 1: 500000. SAGyP-INTA, Argentina.
Iriarte, J., & Paz, E. (2009). Phytolith analysis of selected native plants and modern soils from southeastern Uruguay and its implications for paleoenvironmental and archeological reconstruction. Quaternary International, 193, 99–123. https://doi.org/10.1016/j.quaint.2007.10.008
Katz, O. (2015). Silica phytoliths in angiosperms: phylogeny and early evolutionary history. New Phytologist, 208(3), 642–646.
Keller, C., Guntzer, F., Barboni, D., Labreuche, J., & Meunier, J. D. (2012). Impact of agriculture on the Si biogeochemical cycle: Input from phytolith studie. Comptes Rendus Geoscience 344, 739–746. https://doi.org/10.1016/j.crte.2012.10.004
Keller, T., Colombi, T., Ruiz, S., Manalili, M.P., Rek, J., Stadelmann, V., Or, D., et al. (2017). Long-term soil structure observatory for monitoring post-compaction evolution of soil structure. Vadose Zone Jounal, 16(4), 1-16. DOI: 10.2136/vzj2016.11.0118
Labouriau, L. G. (1983). Phytolith work in Brazil: a minireview. The Phytolitharien Newsletter, 2(2), 6–10.
Latorre, F., Fernández Honaine, M., & Osterrieth, M. (2012). First report of phytoliths in the air of Argentina. Aerobiologia, 28, 61–69. https://doi.org/10.1007/s10453-011-9211-5
Li, Z., Unzue-Belmonte, D., Cornelis, J. T., Vander Linden, C., Struyf, E., Ronsse, F., & Delvaux, B. (2019). Effects of phytolithic rice-straw biochar, soil buffering capacity and pH on silicon bioavailability. Plant and Soil, 438, 187–203. https://doi.org/10.1007/s11104-019-04013-0
Liu, Y., Liu, H., Jie, D., Gao, G., Meng, M., & Zhang, G. (2021). Phytolith morphotypes of woody plants and their preservation in soil in the warm temperate humid zones of China. Quaternary International, 599, 158–169.
Liu, H., Meunier, J. D., Grauby, O., Labille, J., Alexandre, A., & Barboni, D. (2023). Dissolution does not affect grass phytolith assemblages. Palaeogeography, Palaeoclimatology, Palaeoecology, 610, 111345. https://doi.org/10.1016/j.palaeo.2022.111345
Madella, M., & Lancelotti, C. (2012). Taphonomy and phytoliths: a usermanual. Quaternary International, 275, 76–83. https://doi.org/10.1016/j.quaint.2011.09.008
Manzoni, M. (2016). El acceso a la tierra en el Sureste de la Provincia de Buenos Aires durante la segunda mitad del siglo XX: el caso de la Colonia agrícola Laguna de los Padres (Bachelor Thesis). Universidad Nacional de Mar del Plata.
Martínez, G. A. (2001). Geomorfología y geología del Cenozoico superior de las cuencas de los arroyos Los Cueros y Seco, vertiente nororiental de las Sierras Septentrionales, provincia de Buenos Aires ( Ph.D. Thesis). Universidad Nacional del Sur.
Martínez, E., Fuentes, J. P., & Acevedo, E. (2008). Carbono orgánico y propiedades del suelo. Journal of Soil Science and Plant Nutrition, 8(1), 68–96. dx.doi.org/10.4067/S0718-27912008000100006
Martiren, V. S., Fonterosa, R. A., Lastra-Bravo, X. B., & Botta, G. F. (2016). Compactación por el tráfico de la maquinaria agrícola: su efecto sobre el esfuerzo cortante del suelo y el rendimiento del cultivo de maíz (Zea mayz L.). Revista Siembra, 3, 021-036. https://doi.org/10.29166/siembra.v3i1.256
Mazzolari, A. C., & Comparatore, V. (2014). Invasion of Rubus ulmifolius (Rosaceae) in Laguna de los Padres Natural Reserve, Buenos Aires, Argentina: basis for drawing management strategies and recovery of native forests. BioScriba, 7(1), 19–29.
Morris, L. R., Baker, F. A., Morris, C., & Ryel, R. J. (2009). Phytolith types and type-frequencies in native and introduced species of the sagebrush steppe and pinyon–juniper woodlands of the Great Basin, USA. Review of Palaeobotany and Palynology, 157(3–4), 339–357. https://doi.org/10.1016/j.revpalbo.2009.06.007
Mwiti, F. M., Gitau, A. N., & Mbuge, D. O. (2022). Edaphic response and behavior of agricultural soils to mechanical perturbation in tillage. AgriEngineering, 4(2), 335–355. https://doi.org/10.3390/agriengineering4020023
Neumann, K., Fahmy, A.G., Müller-Scheeßel, N., & Schmidt, M., 2017. Taxonomic, ecological and palaeoecological significance of leaf phytoliths in West African grasses. Quaternary International, 434, 15-32. https://doi.org/10.1016/j.quaint.2015.11.039
Nguyen, M.N., Dultz, S., Kasbohm, J., & Le, D. (2009). Clay dispersion and its relation to surface charge in a paddy soil of the Red River Delta, Vietnam. Journal of Soil Science and Plant Nutrition, 172(4), 477–486. https://doi.org/10.1002/jpln.200700217
Nguyen, M. N., Dultz, S., Meharg, A., Pham, Q. V., Hoang, A.N., Dam, T. N. T., Nguyen, V. T., Nguyen, K. M.. Nguyen, H. X., & Nguyen, N. T. (2019). Phytolith content in Vietnamese paddy soils in relation to soil properties. Geoderma, 333, 200–213. https://doi.org/10.1016/j.geoderma.2018.07.027
Novello, A., Bamford, M. K., van Wijk, Y., & Wurz, S. (2018). Phytoliths in modern plants and soils from Klasies River, cape region (South Africa). Quaternary International, 464, 440–459. https://doi.org/10.1016/j.quaint.2017.10.009
Osterrieth, M. (2004). Mineralogía y micromorfología de suelos y paleosuelos de la llanura fluvioeólica bonaerense. Actas XIX Congreso Argentino de la Ciencia del Suelo. 351.
Osterrieth, M. (2006). Silicofitolitos en suelos, paleosuelos y sedimentos. Actas III Congreso Argentino de Cuaternario y Geomorfología, I.
Osterrieth, M. L., & Martínez, G. A. (1993). Paleosols on Late Cainozoic loessic sequences in the northeastern side of Tandilia Range, Buenos Aires, Argentina. Quaternary International, 17, 57–65.
Osterrieth, M., Madella, M., Zurro, D., & Álvarez, M. F. (2009). Taphonomical aspects of silicophytoliths in the loess sediments of the Argentinean Pampas. Quaternary International, 193, 70–79 https://doi.org/10.1016/j.quaint.2007.09.002
Osterrieth, M., Fernández Honaine, M., Borrelli, N., & Alvarez, M. F., (2014). Silicophytoliths in representative soils of the southeast Pampean Plains, Argentina. In H., Gomes Coe, & Osterrieth, M. (Eds.), Synthesis of some phytolith studies in South America (Brazil and Argentina) (pp. 215-242). Nova Science Publishers, Inc.
Osterrieth, M., Borrelli, N., Álvarez, M.F., & Fernández Honaine, M. (2015). Silica biogeochemical cycle in temperate ecosystems of the pampean plain, Argentina. Journal of South American Earth Sciences, 172–79. https://doi.org/10.1016/j.jsames.2015.07.011
Pan, W., Song, Z., Liu, H., Müeller, K., Yang, X., Zhang, X., Li, Z., Liu, X., Qiu, S., Hao, Q., & Wang, H. (2017). Impact of grassland degradation on soil phytolith carbon sequestration in Inner Mongolian steppe of China. Geoderma, 308, 86–92.
Paolicchi, M., (2022). Incorporación, contenido y tafonomía de silicofitolitos en suelos asociados a actividad agrícola-ganadera del sudeste bonaerense (Ph. D. thesis). Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Argentina.
Paolicchi, M., Fernández Honaine, M., & Osterrieth, M. (2021). Herbivore effect in the assemblages of phytoliths incorporated to soils from cow dung. Review of Palaeobotany and Palynology, 288, 104401. https://doi.org/10.1016/j.revpalbo.2021.104401
Pearsall, D.M., Chandler-Ezell, K., & Chandler-Ezell, A. (2003). Identificación de maíz en sedimentos y suelos neotropicales utilizando fitolitos de mazorca. Revista de Ciencias Arqueológicas, 30(5), 611–627.
Piperno, D. R. (2006). Phytoliths: A Comprehensive Guide for Archaeologists and Paleoecologists. San Diego: AltaMira Press
Premathilake, R., Akhilesh, K., Anupama, K., Prasad, S., Gunnell, Y., Orukaimani, G., & Pappu, S. (2022). Issues of phytolith taphonomy at Palaeolithic sites: Investigation and results from Attirampakkam, India. Journal of Archaeological Science: Reports, 42, 103357.
Qader, W., Mir, S.H., Meister, J., Dar, R.A., Madella, M., & Rashid, I. (2023). Sedimentological perspective on phytolith analysis in palaeoecological reconstruction. Earth Science Reviews, 104549. https://doi.org/10.1016/j.earscirev.2023.104549
R Core Team (2024). R: A language and environment for statistical computing [Computer software]. R Foundation for Statistical Computing. Vienna, Austria.
Schaller, J., Puppe, D., Kaczorek, D., Ellerbrock, R., & Sommer, M. (2021). Silicon cycling in soils revisited. Plants, 10(2), 295.
Servicio Meteorológico Nacional (2010). Estadísticas climatológicas. Publicaciones del Servicio Meteorológico Nacional, Buenos Aires, Argentina.
Shahack-Gross, R. (2011). Herbivorous livestock dung: Formation, taphonomy, methods for identification, and archaeological significance. Journal of Archaeological Science, 38, 205–218. https://doi.org/10.1016/j.jas.2010.09.019
Soil Survey Staff (1996). Keys to Soil Taxonomy. 7th edition, Departament of Agriculture, USA.
Sommer, M., Kaczorek, D., Kuzyakov, Y., & Breuer, J. (2006). Silicon pools and fluxes in soils and landscapes—a review. Journal of Plant Nutrition and Soil Science, 169(3), 310-329.
Sommer, M., Jochheim, H., Höhn, A., Breuer, J., Zagorski, Z., Busse, J., Barkusky, D., Meier, K., Puppe, D., Wanner, M., & Kaczorek, D. (2013). Si cycling in a forest biogeosystem–the importance of transient state biogenic Si pools. Biogeosciences, 10(7), 4991–5007.
Soriano, A., Leon, R. J. C., Sala, O. E., Lavado, R. S., Deregibus, V. A., Cauhepe, M. A., & Lemcoff, J.H. (1991). Río de la Plata Grasslands. In R. T. Coupland (Ed.), Natural grasslands. Introduction and Western Hemisphere. Ecosystem of the world (pp. 367–407). New York, Elsevier.
Strömberg, C. A. E., Di Stilio, V.S., & Song, Z. (2016). Functions of phytoliths in vascular plants: an evolutionary perspective. Functional Ecology, 30(8), 1286–1297. https://doi.org/10.1111/1365-2435.12692
Thorn, V. C. (2004). Phytoliths from subantarctic Campbell Island: plant production and soil surface spectra. Review of Palaeobotany and Palynology, 132, 37–59. https://doi.org/10.1016/j.revpalbo.2004.04.003
Tubana, B., Tapasya, B., & Datnoff, E. L. (2016). A review of silicon in soil in plants and its role on US agriculture: history and future perspective. Soil science, 181(9–10), 393–411. https://doi.org/10.1097/SS.0000000000000179
Vandevenne, F., Struyf, E., Clymans, W., & Meire, P. (2012). Agricultural silica harvest: Have humans created a new loop in the global silica cycle?. Frontiers in Ecology and the Environment, 10, 243–248.
Vandevenne, F.I., Barão, A.L., Schoelynck, J., Smis, A., Ryken, N., Van Damme, S., Meire, P. & Struyf, E. (2013).Grazers: biocatalysts of terrestrial silica cycling. Proceedings of the Royal Society B: Biological Sciences, 280(1772), 20132083.
Walkley, A., & Black, C. A. (1965). Organic carbon. In C. Black, (Ed.), Methods of Soil Analysis (1372–1375). American Society of Agronomy.
Wallis, L. A. (2001). Environmental history of northwest Australia based on phytolith analysis at Carpenter’s Gap 1. Quaternary International, 83–85, 103–117. https://doi.org/10.1016/S1040-6182(01)00033-7
Wallis, L. A. (2003). An overview of leaf phytolith production patterns in selected northwest Australian flora. Review of Palaeobotany and Palynology, 125, 201–248. https://doi.org/10.1016/S0034-6667(03)00003-4
Yang, C., Zhang, Y., Hou, F., Millner, J., Wang, Z., & Chang, S. (2019). Grazing activity increases decomposition of yak dung and litter in an alpine meadow on the Qinghai-Tibet plateau. Planta and Soil, 444, 239–250. https://doi.org/10.1007/s11104-019-04272-x

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